DNA Sequencing Sample Preparation Guidelines and Pricing

(Updated December 1, 2011)

All sample preps should only contain, DNA, primer (if added), and water. Please avoid using any buffers, which can interfere with the sequencing reaction, purification, and injection on the instrument ultimately diminishing data quality. Qiagen mini or maxi preps are recommended purification kits.

NEW PRICING: Primer Added $6 per rxn for orders over 16 samples and $4 per rxn for orders over 64 samples (price details below). Please submit orders over 16 samples in strip clearly labelled strip tubes or 96-well plates.


 

Inside the Institute /CFAR

Outside the Institute

Primer Added

< 16 Samples

$8.00

$8.00

16-64 Samples

$6.00

$6.00

> 64 Samples

$4.00

$6.00

Primer NOT Added

$8.00

$10.00

Difficult Template

$12.00

$15.00


Primer Added Fast Track (you add primer)

Plasmid
50 ng/ul DNA
3.2 uM primer already added
>12ul volume of DNA plus primer per reaction (we need at least 600ng of DNA per reaction)

PCR
5ng/ul DNA
3.2 uM primer already added
10ul per reaction (we need at least 25ng of DNA per reaction)

**Please submit orders over 16 samples in strip clearly labelled strip tubes or 96-well plates**


Primer NOT Added Fast Track (we add primer)

Facility Primers

Plasmid
50 ng/ul DNA
>12ul per reaction (we need at least 600ng of DNA per reaction)
*Primer is NOT already added and either supplied in a separate tube or stored at MBCF

PCR
5ng/ul DNA
10ul per reaction (we need at least 25ng of DNA per reaction)
*Primer is NOT already added and either supplied in a separate tube or stored at MBCF

Need a Custom Oligo? Click here to login and place an order through our Oligosyn LIMS.


Difficult Template

This service is recommended for those wishing to sequence hairpins, GC-rich templates, or any sample that is being "difficult" and requires special attention. Difficult Template samples require additional information that should be supplied in the comments section of the online order form.

Plasmid
50 ng/ul DNA
3.2 uM primer (either premixed or supplied separately)
>>12ul per reaction (please give us enough to repeat reaction if necessary)

PCR
5ng/ul DNA
3.2 uM primer (either premixed or supplied separately)
10ul per reaction (please give us enough to repeat reaction if necessary)

BAC (must be submitted as difficult template)
1.0 ug/ul DNA (>10ul per reaction)
10uM primer (submitted separately)
*Please be sure to include additional information in the comments section of the online order form indicating why your sample is "difficult."

Questions? Email Zach or Amy or call us 617-632-3082.